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> 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E07286h] Human estrogen,E ELISA Kit
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[CSB-E07286h] Human estrogen,E ELISA Kit

제품코드 : CSB-E07286h

제품정보 : Human estrogen,E

  • Sample Types: serum, plasma
  • Detect Range: 25 pg/mL-1000 pg/mL
  • Sensitivity: 25 pg/mL

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  • 전화번호
    2292-8870

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Product Details

Description

This Human estrogen (E) ELISA Kit was designed for the quantitative measurement of Human estrogen (E) protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 25 pg/mL-1000 pg/mL and the sensitivity is 25 pg/mL.

Alternative NamesN/A
AbbreviationE
SpeciesHomo sapiens (Human)
Sample Typesserum, plasma
Detection Range25 pg/mL-1000 pg/mL
Sensitivity25 pg/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaSignal Transduction
Assay Principlequantitative
MeasurementCompetitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%   
Three samples of known concentration were tested twenty times on one plate to assess. 
Inter-assay Precision (Precision between assays): CV%<15%   
Three samples of known concentration were tested in twenty assays to assess.  
       
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human estrogen in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4) 
1:1Average %95 
Range %88-101 
1:2Average %94 
Range %86-100 
1:4Average %97 
Range %94-101 
1:8Average %91 
Range %85-96 
Recovery
The recovery of human estrogen spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange 
Serum (n=5)9087-94 
EDTA plasma (n=4)9692-100 
       
       
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2Average  
02.3912.3512.371  
251.5421.5391.541  
750.8650.8420.854  
2300.5670.5650.566  
5000.3610.3590.360  
10000.1560.1530.155  
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days