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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [SEC057Hu] ELISA Kit for Receptor Activator Of Nuclear Factor Kappa B (RANk)

[SEC057Hu] ELISA Kit for Receptor Activator Of Nuclear Factor Kappa B (RANk)

제품코드 : SEC057Hu

제품정보 : Signal transduction;Metabolic pathway;Bone metabolism;

Organism Species: Homo sapiens (Human)
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Test Method: Double-antibody Sandwich
Assay Length: 3h
Detection Range: 0.156-10ng/mL
Sensitivity: The minimum detectable dose of this kit is typically less than 0.058ng/mL

제품문의

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

제품상세

Specificity

This assay has high sensitivity and excellent specificity for detection of Receptor Activator Of Nuclear Factor Kappa B (RANk).
No significant cross-reactivity or interference between Receptor Activator Of Nuclear Factor Kappa B (RANk) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Receptor Activator Of Nuclear Factor Kappa B (RANk) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Receptor Activator Of Nuclear Factor Kappa B (RANk) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×120µL	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
TMB Substrate	1×9mL	Stop Solution	1×6mL
Wash Buffer (30 × concentrate)	1×20mL	Instruction manual	1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.