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> 제품정보 > 시약 > 해외 공식 대리점 > [1417-010] Truncated Human TPO, accession # P40225, Ser22-Leu195
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[1417-010] Truncated Human TPO, accession # P40225, Ser22-Leu195

제품코드 : 1417-010

제품정보 : CellGenix® Recombinant Human TPO reliably supports the self-renewal and maintenance of hematopoietic stem cells (HSCs) as well as the differentiation of HSCs into natural killer cells (NK cells). It is also used to differentiate embryonic stem cells (ESCs

  • Produced in our dedicated animal-free facility
  • Compliant to USP Chapter <1043> and Ph. Eur. General Chapter 5.2.12 (GMP)
  • FDA Drug Master File available (GMP)

제품문의

  • 전화번호
    2292-8870

제품상세

General Information

Thrombopoietin (TPO) is a glycoprotein hormone produced by the liver and kidney. It regulates the production of platelets and stimulates the production and differentiation of megakaryocytes.

TPO is used in the cell and gene therapy space for the ex vivo expansion and differentiation of HSCs.


Technical Details

Source
Expressed in E. coli
Description

Truncated Human TPO, accession # P40225, Ser22-Leu195

Formulation

Lyophilized from a 0.2 µm-filtered aqueous solution

Both product grades are produced under the same conditions in a GMP facility, ensuring an equal product quality and performance. We offer a more comprehensive QC testing including tighter specifications and documentation for our GMP products: Preclinical vs GMP.

 PreclinicalGMP
Molecular weight19.6 kDa19.6 kDa
Purity≥ 95% as determined by SDS-PAGE≥ 98% as determined by SDS-PAGE
Activity≥ 10 x 106 IU/mg, calibrated against an in-house reference standard
Measured in a cell proliferation assay using a TPO-dependent cell line, MO7e
10 – 40 x 106 IU/mg, calibrated against an in-house reference standard
Measured in a cell proliferation assay using a TPO-dependent cell line, MO7e

Batch specific activity on CoA

Endotoxin level< 1000 EU/mg≤ 50 EU/mg
Intended useIntended for preclinical ex vivo use. Not intended for therapeutic use.Intended for clinical ex vivo use. Not intended for human in vivo application.

Handling Instructions

Reconstitution

Recommended in sterile water to a final concentration of 250 µg/ml for 50 µg vials or 500 µg/ml for 1 mg vials.

Shipment

Ambient temperature. Please refer to our Technote Shipment of CellGenix® Preclinical and GMP Cytokines at Ambient Temperatures.

Expiry

≥ 6 months from date of shipping. Please refer to Certificate of Analysis for the exact expiry date.

Storage & Stability

Store lyophilized cytokine at -20°C to -80°C.

Store a 250 µg/ml reconstituted cytokine solution:

• 4 weeks at 2°C to 8°C under sterile conditions after reconstitution. Store in the original container. 

• 4 months at -20°C to -80°C under sterile conditions after reconstitution. Store in 60 µl aliquots in polypropylene cryogenic vials.

Avoid repeated freeze/thaw cycles.