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> 제품정보 > 시약 > 해외 공식 대리점 > [1414-010] Human IL-10, accession # P22301, Ser19-Asn178
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[1414-010] Human IL-10, accession # P22301, Ser19-Asn178

제품코드 : 1414-010

제품정보 : CellGenix Recombinant Human IL-10 reliably promotes the generation of anti-inflammatory dendritic cells (DCs). CellGenix rh IL-10 is produced in our dedicated animal-free GMP facility ensuring maximum safety for optimal use in ATMP manufacturing.

  • Produced in our dedicated animal-free facility
  • Compliant to USP Chapter <1043> and Ph. Eur. General Chapter 5.2.12 (GMP)
  • FDA Drug Master File available (GMP)

제품문의

  • 전화번호
    2292-8870

제품상세

General Information

Interleukin-10 (IL-10) is an anti-inflammatory cytokine, which is classified as a class-2 cytokine. It amongst others down regulates the expression of T helper 1 cytokines, MHC Class II antigens, and co-stimulatory molecules on macrophages.

IL-10 is used in the cell and gene therapy space to support the generation of anti-inflammatory DCs.


Technical Details

Source
Expressed in E. coli
Description

Human IL-10, accession # P22301, Ser19-Asn178

Formulation

Lyophilized from 200µl of a 0.2 µm-filtered solution containing 1.5 mM potassium phosphate, 8,1 mM sodium phosphate, 2.7 mM potassium chloride and 137 mM sodium chloride, pH 7.5

Both product grades are produced under the same conditions in a GMP facility, ensuring an equal product quality and performance. We offer a more comprehensive QC testing including tighter specifications and documentation for our GMP products: Preclinical vs GMP.

 PreclinicalGMP
Molecular weight19.6 kDa19.6 kDa
Purity≥ 95% as determined by SDS-PAGE≥ 97% as determined by SDS-PAGE
Activity≥ 2.5 x 106 IU/mg, calibrated against NIBSC #93/722
Measured in a cell proliferation assay using an IL-10 sensitive cell line, MC/9
3 – 50 x 106 IU/mg, calibrated against NIBSC #93/722
Measured in a cell proliferation assay using an IL-10-dependent cell line, MC/9

Batch specific activity on CoA

Endotoxin level< 1000 EU/mg≤ 50 EU/mg
Intended useIntended for preclinical ex vivo use. Not intended for therapeutic use.Intended for clinical ex vivo use. Not intended for human in vivo application.

 

Handling Instructions

Reconstitution

Recommended in sterile water to a final concentration of 250 µg/ml.

Shipment

Ambient temperature. Please refer to our Technote Shipment of CellGenix® Preclinical and GMP Cytokines at Ambient Temperatures.

Expiry

≥ 6 months from date of shipping. Please refer to Certificate of Analysis for the exact expiry date.

Storage & Stability

Store lyophilized cytokine at -20°C to -80°C.

Avoid repeated freeze/thaw cycles.