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> 제품정보 > 시약 > 해외 공식 대리점 > [1421-050] Human FGF-2, accession # P090938, Ala135-Ser288
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[1421-050] Human FGF-2, accession # P090938, Ala135-Ser288

제품코드 : 1421-050

제품정보 : CellGenix® Recombinant Human FGF-2, also known as bFGF, reliable improves proliferation of mesenchymal stem cells (MSCs). It in addition supports proliferation and differentiation of chondrocytes and supports propagation of undifferentiated pluripotent st

  • Contains neither animal- nor human-derived materials
  • Compliant to USP Chapter <1043> and Ph. Eur. General Chapter 5.2.12 (GMP)
  • FDA Drug Master File available (GMP)

제품문의

  • 전화번호
    2292-8870

제품상세

General Information

Fibroblast Growth Factor-2 (FGF-2) is a basic fibroblast growth factor. It amongst others plays an important role in wound healing and tumor development by mediating the formation of new blood vessels.

FGF-2 is used in the cell and gene therapy space for the expansion of bone marrow and adipose tissue derived MSCs.


Technical Details

Source
Expressed in E. coli
Description

Human FGF-2, accession # P090938, Ala135-Ser288

Formulation

Lyophilized from a 0.2 µm-filtered solution containing 25 mM sodium phosphate, 400 mM sodium chloride, 10 mM glutathione (red.) and 5% mannitol, pH 6.5

Both product grades are produced under the same conditions in a GMP facility, ensuring an equal product quality and performance. We offer a more comprehensive QC testing including tighter specifications and documentation for our GMP products: Preclinical vs GMP.

 PreclinicalGMP
Molecular weight17.3 kDa17.3 kDa
Purity≥ 95 % as determined by SDS-PAGE≥ 97 % as determined by SDS-PAGE and RP-HPLC
Activity≥ 0.9 x 106 IU/mg, calibrated against NIBSC #90/712
Measured in a cell proliferation assay using a FGF-2-dependent cell line, FBHE
0.9 – 3 x 106 IU/mg, calibrated against NIBSC #90/712
Measured in a cell proliferation assay using a FGF-2-dependent cell line, FBHE

Batch specific activity on CoA

Endotoxin level< 1000 EU/mg≤ 50 EU/mg
Intended useIntended for preclinical ex vivo use. Not intended for therapeutic use.Intended for clinical ex vivo use. Not intended for human in vivo application.

Handling Instructions

Reconstitution

Recommended in sterile water to a final concentration of 250 µg/ml for 50 µg vials or 500 µg/ml for 1 mg vials.

Shipment

Ambient temperature. Please refer to our Technote Shipment of CellGenix® Preclinical and GMP Cytokines at Ambient Temperatures.

Expiry

≥ 6 months from date of shipping. Please refer to Certificate of Analysis for the exact expiry date.

Storage & Stability

Store lyophilized cytokine at -20°C to -80°C.

Store a 250 µg/ml reconstituted cytokine solution:

• 4 months at -20°C to -80°C under sterile conditions after reconstitution. Store in 60 µl aliquots in polypropylene cryogenic vials.

Avoid repeated freeze/thaw cycles.