본문 바로가기 주메뉴 바로가기

인류의 건강과 생명공학 발전을 위해 봉사하는 기업 드림셀

We Serving the Health and Biotechnology of Humanity

제품정보

> 제품정보 > 시약 > 해외 공식 대리점 > [1418-050] Human SCF, accession # P21583, Glu26-Ala189
제품명

[1418-050] Human SCF, accession # P21583, Glu26-Ala189

제품코드 : 1418-050

제품정보 : CellGenix® Recombinant Human SCF reliably supports survival and differentiation of hematopoietic stem cells (HSCs) and progenitor cells as well as the differentiation of cord blood-derived CD34+ cells into natural killer cells (NK cells). It is also used

  • Produced in our dedicated animal-free facility
  • Compliant to USP Chapter <1043> and Ph. Eur. General Chapter 5.2.12 (GMP)
  • FDA Drug Master File available (GMP)

제품문의

  • 전화번호
    2292-8870

제품상세

General Information

Stem Cell Factor (SCF) is a cytokine that is produced by fibroblasts and endothelial cells. It plays an important role in hematopoiesis, spermatogenesis and melanogenesis.

SCF is used in the cell and gene therapy space for the expansion of HSCs that are used for transplantation.


Technical Details

Source
Expressed in E. coli
Description

Human SCF, accession # P21583, Glu26-Ala189

Formulation

Lyophilized from a 0.2 µm-filtered solution containing 25 mM sodium phosphate, 150 mM sodium chloride and 1 mM EDTA, pH 7.4

Both product grades are produced under the same conditions in a GMP facility, ensuring an equal product quality and performance. We offer a more comprehensive QC testing including tighter specifications and documentation for our GMP products: Preclinical vs GMP.

 PreclinicalGMP
Molecular weight19.4 kDa19.4 kDa
Purity≥ 95% as determined by SDS-PAGE≥ 97% as determined by SDS-PAGE
Activity≥ 0.5 x 106 IU/mg, calibrated against NIBSC #91/682 Measured in a cell proliferation assay using a SCF-dependent cell line, TF-10.5 – 2 x 106 IU/mg, calibrated against NIBSC #91/682 Measured in a cell proliferation assay using a SCF-dependent cell line, TF-1

Batch specific activity on CoA

Endotoxin level< 1000 EU/mg≤ 50 EU/mg
Intended useIntended for preclinical ex vivo use. Not intended for therapeutic use.Intended for clinical ex vivo use. Not intended for human in vivo application.

Handling Instructions

Reconstitution

Recommended in sterile water to a final concentration of 250 µg/ml for 50 µg vials or 500 µg/ml for 1 mg vials.

Shipment

Ambient temperature. Please refer to our Technote Shipment of CellGenix® Preclinical and GMP Cytokines at Ambient Temperatures.

Expiry

≥ 6 months from date of shipping. Please refer to Certificate of Analysis for the exact expiry date.

Storage & Stability

Store lyophilized cytokine at -20°C to -80°C.

Store a 250 µg/ml reconstituted cytokine solution:

• 4 weeks at 2°C to 8°C under sterile conditions after reconstitution. Store in the original container. 

• 4 months at -20°C to -80°C under sterile conditions after reconstitution. Store in 60 µl aliquots in polypropylene cryogenic vials.

Avoid repeated freeze/thaw cycles.