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> 제품정보 > 시약 > 해외 공식 대리점 > [1409-050] Human PDGF-BB, accession # P01127, Ser82-Thr190
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[1409-050] Human PDGF-BB, accession # P01127, Ser82-Thr190

제품코드 : 1409-050

제품정보 : CellGenix® Recombinant Human PDGF-BB reliably supports proliferation of mesenchymal stem cells (MSCs) and chondrocytes. CellGenix® rh PDGF-BB is produced in our dedicated animal-free GMP facility ensuring maximum safety for optimal use in ATMP manufacturi

  • Produced in our dedicated animal-free facility
  • Compliant to USP Chapter <1043> and Ph. Eur. General Chapter 5.2.12 (GMP)

제품문의

  • 전화번호
    2292-8870

제품상세

General Information

Platelet-derived growth factor-BB (PDGF-BB) is a member of the platelet-derived growth factor family. It is produced by platelets and plays an important role in angiogenesis.

PDGF-BB is used in the cell and gene therapy space for the expansion of bone marrow and adipose tissue derived MSCs and chondroctyes.


Technical Details

Source
Expressed in E. coli
Description

Human PDGF-BB, accession # P01127, Ser82-Thr190

Formulation

Lyophilized from a 0.2 µm-filtered solution containing 1.5 mM potassium phosphate, 8.1 mM sodium phosphate, 2.7 mM potassium chloride and 137 mM sodium chloride, pH 7.5

Both product grades are produced under the same conditions in a GMP facility, ensuring an equal product quality and performance. We offer a more comprehensive QC testing including tighter specifications and documentation for our GMP products: Preclinical vs GMP.

 PreclinicalGMP
Molecular weight24.8 kDa24.8 kDa
Purity≥ 95% as determined by SDS-PAGE≥ 97% as determined by SDS-PAGE
Activity≥ 0.4 x 106 IU/mg, calibrated against NIBSC #94/728 Measured in a cell proliferateon assay using a PDGF-BB-dependent cell line, Balb/3T31 – 4 x 106 IU/mg, calibrated against NIBSC #94/728 Measured in a cell proliferation assay using a PDGF-BB-dependent cell line, Balb/3T3

Batch specific activity on CoA

 

Endotoxin level< 1000 EU/mg≤ 50 EU/mg
Intended useIntended for preclinical ex vivo use. Not intended for therapeutic use.Intended for clinical ex vivo use. Not intended for human in vivo application.

Handling Instructions

Reconstitution

Recommended in sterile water to a final concentration of 250 µg/ml for 50 µg vials or 500 µg/ml for 1 mg vials.

Shipment

Ambient temperature. Please refer to our Technote Shipment of CellGenix® Preclinical and GMP Cytokines at Ambient Temperatures.

Expiry

≥ 6 months from date of shipping. Please refer to Certificate of Analysis for the exact expiry date.

Storage & Stability

Store lyophilized cytokine at -20°C to -80°C.

Store a 250 µg/ml reconstituted cytokine solution:

• 4 weeks at 2°C to 8°C under sterile conditions after reconstitution. Store in the original container. 

• 4 months at -20°C to -80°C under sterile conditions after reconstitution. Store in 60 µl aliquots in polypropylene cryogenic vials.

Avoid repeated freeze/thaw cycles.