본문 바로가기 주메뉴 바로가기

인류의 건강과 생명공학 발전을 위해 봉사하는 기업 드림셀

We Serving the Health and Biotechnology of Humanity

제품정보

> 제품정보 > 시약 > 해외 공식 대리점 > [1406-050] Human TNF-α, accession # P01375, Val77-Leu233
제품명

[1406-050] Human TNF-α, accession # P01375, Val77-Leu233

제품코드 : 1406-050

제품정보 : CellGenix® Recombinant Human TNF-α reliably stimulates the maturation of immature dendritic cells (DCs). CellGenix® rh TNF-α is produced in our dedicated animal-free GMP facility ensuring maximum safety for optimal use in ATMP manufacturing.

  • Produced in our dedicated animal-free facility
  • Compliant to USP Chapter <1043> and Ph. Eur. General Chapter 5.2.12 (GMP)
  • FDA Drug Master File available (GMP)

제품문의

  • 전화번호
    2292-8870

제품상세

General Information

Tumor necrosis factor alpha (TNF-α) is a cytokine that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. It is mainly produced by monocytes and macrophages as a response to infection, injury, or tumor burden. TNF-α can also be produced from a range of other cell types such as T cells, natural killer cells (NK cells) and neutrophils.

CellGenix® TNF-α is used in the cell and gene therapy space for the ex vivo maturation of immature DCs.


Technical Details

Source
Expressed in E. coli
Description

Human TNF-α, accession # P01375, Val77-Leu233

Formulation

Lyophilized from 200 µl of a 0.2 µm-filtered solution containing 1.5 mM potassium phosphate, 8.1 mM sodium phosphate, 2.7 mM potassium chloride and 137 mM sodium chloride, pH 7.5

Both product grades are produced under the same conditions in a GMP facility, ensuring an equal product quality and performance. We offer a more comprehensive QC testing including tighter specifications and documentation for our GMP products: Preclinical vs GMP.

 PreclinicalGMP
Molecular weight17.5 kDa17.5 kDa
Purity≥ 95% as determined by SDS-PAGE≥ 97% as determined by SDS-PAGE
Activity≥ 20 x 106 IU/mg, calibrated against NIBSC #88/786
Measured in a cell cytotoxicity assay using a TNF-α-sensitive cell line, L929

20 – 80 x 106 IU/mg, calibrated against NIBSC #88/786
Measured in a cell cytotoxicity assay using a TNF-α-sensitive cell line, L929

Batch specific activity on CoA

Endotoxin level< 1000 EU/mg≤ 50 EU/mg
Intended useIntended for preclinical ex vivo use. Not intended for therapeutic use.Intended for clinical ex vivo use. Not intended for human in vivo application.

 

Handling Instructions

Reconstitution

Recommended in sterile water to a final concentration of 250 µg/ml.

Shipment

Ambient temperature. Please refer to our Technote Shipment of CellGenix® Preclinical and GMP Cytokines at Ambient Temperatures.

Expiry

≥ 6 months from date of shipping. Please refer to Certificate of Analysis for the exact expiry date.

Storage & Stability

Store lyophilized cytokine at -20°C to -80°C.

Store a 100 µg/ml reconstituted cytokine solution:

• 4 weeks at 2°C to 8°C under sterile conditions after reconstitution. Store in the original container. 

• 4 months at -20°C to -80°C under sterile conditions after reconstitution. Store in 60 µl aliquots in polypropylene cryogenic vials.

Avoid repeated freeze/thaw cycles.