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> 제품정보 > 시약 > 해외 공식 대리점 > [1020-050] Human IL-2 with a substitution of cysteine-125 by serine, accession # P60568, Pro22-Thr153, N-terminal Met and C-terminal 6xHis-tag, molecular mass 16.3 kDa
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[1020-050] Human IL-2 with a substitution of cysteine-125 by serine, accession # P60568, Pro22-Thr153, N-terminal Met and C-terminal 6xHis-tag, molecular mass 16.3 kDa

제품코드 : 1020-050

제품정보 : CellGenix® Recombinant Human IL-2 reliably activates and expands T cells, natural killer cells (NK cells) and cytokine-induced killer cells (CIK cells). CellGenix® rh IL-2 is produced in our dedicated animal-free GMP facility ensuring maximum safety for o

  • Produced in our dedicated animal-free facility
  • Compliant to USP Chapter <1043> and Ph. Eur. General Chapter 5.2.12 (GMP)
  • FDA Drug Master File available (GMP)
  • Available in different formats (lyophilized & liquid)

제품문의

  • 전화번호
    2292-8870

제품상세

General Information

Interleukin-2 (IL-2) is a cytokine signaling molecule that is mainly produced by T cells. It is a central regulator of immune responses and plays a role in anti-inflammatory reactions, hematopoiesis and tumor surveillance.

IL-2 is used in the cell and gene therapy space to promote proliferation and differentiation of CAR T cells, TCR T cells, Tregs, TILs, NK cells and CIK cells.


Technical Details

Source
Expressed in E. coli
Description

Human IL-2 with a substitution of cysteine-125 by serine, accession # P60568, Pro22-Thr153, N-terminal Met and C-terminal 6xHis-tag, molecular mass 16.3 kDa.

With the exception of the added C-terminal 6xHis-tag, the amino acid sequence corresponds to that of Aldesleukin.

Formulation

Lyophilized from a 0.2 µm-filtered solution containing 25 mM sodium acetate and 50 mM sodium chloride, pH 5.0

Both product grades are produced under the same conditions in a GMP facility, ensuring an equal product quality and performance. We offer a more comprehensive QC testing including tighter specifications and documentation for our GMP products: Preclinical vs GMP.

 
 PreclinicalGMP
Molecular weight16.3 kDa16.3 kDa
Purity≥ 95% as determined by SDS-PAGE and HPLC≥ 97% as determined by SDS-PAGE and HPLC
Activity≥ 8 x 106 IU/mg, calibrated against Proleukin®
Measured in a cell proliferation assay using an IL-2-dependent cell line, CTLL-2
8 – 32 x 106 IU/mg, calibrated against Proleukin®
Measured in a cell proliferation assay using an IL-2-dependent cell line, CTLL-2

Batch specific activity on CoA

Endotoxin level< 25 EU/mg≤ 25 EU/mg
Intended useIntended for preclinical ex vivo use. Not intended for therapeutic use.Intended for clinical ex vivo use. Not intended for human in vivo application.

Handling Instructions

Reconstitution

Recommended in 0.2% acetic acid to a final concentration of 250 µg/ml for 50 µg vials or 500 µg/ml for 1 mg vials.

Shipment

Ambient temperature. Please refer to our Technote Shipment of CellGenix® Preclinical and GMP Cytokines at Ambient Temperatures.

Expiry

≥ 6 months from date of shipping. Please refer to Certificate of Analysis for the exact expiry date.

Storage & Stability

Store lyophilized cytokine at -20°C to -80°C.

Store a 250 µg/ml reconstituted cytokine solution:

• 4 weeks at 2°C to 8°C under sterile conditions after reconstitution. Store in the original container. 

• 4 months at -20°C to -80°C under sterile conditions after reconstitution. Store in 60 µl aliquots in polypropylene cryogenic vials.

Avoid repeated freeze/thaw cycles.