본문 바로가기 주메뉴 바로가기

인류의 건강과 생명공학 발전을 위해 봉사하는 기업 드림셀

We Serving the Health and Biotechnology of Humanity

제품정보

> 제품정보 > 시약 > 해외 공식 대리점 > [1016-1000] Human EGF, accession # P01133, Asn971-Arg1023
제품명

[1016-1000] Human EGF, accession # P01133, Asn971-Arg1023

제품코드 : 1016-1000

제품정보 : CellGenix® Recombinant Human EGF reliably promotes the expansion of mesenchymal stem cells (MSCs). It in addition promotes the differentiation of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs)-derived hepatoblasts. CellGenix® rh EG

  • Produced in our dedicated animal-free facility
  • Compliant to USP Chapter <1043> and Ph. Eur. General Chapter 5.2.12 (GMP)
  • FDA Drug Master File available (GMP)

제품문의

  • 전화번호
    2292-8870

제품상세

General Information

Epidermal Growth Factor (EGF) is a growth factor that promotes cell growth, proliferation and differentiation. It is found in a variety of human body fluids.

EGF is used in the cell and gene therapy space for the expansion of bone marrow and adipose tissue derived MSCs, and the generation of stem cell derived hepatocytes.


Technical Details

Source
Expressed in E. coli
Description

Human EGF, accession # P01133, Asn971-Arg1023

Formulation

Lyophilized from a 0.2 µm-filtered solution containing 25 mM sodium acetate, pH 5.0

Both product grades are produced under the same conditions in a GMP facility, ensuring an equal product quality and performance. We offer a more comprehensive QC testing including tighter specifications and documentation for our GMP products: Preclinical vs GMP.

 
 PreclinicalGMP
Molecular weight7.2 kDa7.2 kDa
Purity≥ 95% as determined by RP-HPLC≥ 97% as determined by RP-HPLC
Activity≥ 0.5 x 106 IU/mg, calibrated against NIBSC #91/530
Measured in a cell proliferation assay using an EGF-dependent cell line, Balb/3T3
0.5 – 2.0 x 106 IU/mg, calibrated against NIBSC #91/530
Measured in a cell proliferation assay using an EGF-dependent cell line, Balb/3T3

Batch specific activity on CoA

Endotoxin level< 1000 EU/mg≤ 50 EU/mg
Intended useIntended for preclinical ex vivo use. Not intended for therapeutic use.Intended for clinical ex vivo use. Not intended for human in vivo application.

Handling Instructions

Reconstitution

Recommended in 0.2% acetic acid to a final concentration of 250 µg/ml for 50 µg vials or 500 µg/ml for 1 mg vials.

Shipment

Ambient temperature. Please refer to our Technote Shipment of CellGenix® Preclinical and GMP Cytokines at Ambient Temperatures.

Expiry

≥ 6 months from date of shipping. Please refer to Certificate of Analysis for the exact expiry date.

Storage & Stability

Store lyophilized cytokine at -20°C to -80°C.

Store a 250 µg/ml cytokine solution

• 4 weeks at 2°C to 8°C under sterile conditions after reconstitution. Store in the original container. 

• 4 months at -20°C to -80°C under sterile conditions after reconstitution. Store in 60 µl aliquots in polypropylene cryogenic vials.

Avoid repeated freeze/thaw cycles.