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> 제품정보 > 시약 > 해외 공식 대리점 > [1002-050] GMP IL-3
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[1002-050] GMP IL-3

제품코드 : 1002-050

제품정보 : CellGenix® Recombinant Human IL-3 reliably stimulates the proliferation of hematopoietic stem cells (HSCs/CD34+ cells). It is produced in our dedicated animal-free GMP facility ensuring maximum safety for optimal use in ATMP manufacturing.

  • Produced in our dedicated animal-free facility
  • Compliant to USP Chapter <1043> and Ph. Eur. General Chapter 5.2.12 (GMP)
  • FDA Drug Master File available (GMP)

제품문의

  • 전화번호
    2292-8870

제품상세

General Information

Interleukin-3 (IL-3) is a cytokine which acts as a mediator in the body’s natural immune system. It amongst others strengthens the immune response by stimulating the proliferation of HSCs into the major blood cell types. IL-3 is produced primarily by activated T cells.

IL-3 is used in the cell and gene therapy space for the ex vivo expansion of HSCs.


Technical Details

Source
Expressed in E. coli
Description

Human IL-3, accession # P08700, Ala20-Phe152

Formulation

Lyophilized from a 200 µl of a 0.2 µm-filtered solution containing 10 mM sodium phosphate and 50 mM sodium chloride, pH 7.2

Both product grades are produced under the same conditions in a GMP facility, ensuring an equal product quality and performance. We offer a more comprehensive QC testing including tighter specifications and documentation for our GMP products: Preclinical vs GMP.

 
 PreclinicalGMP
Molecular weight15.2 kDa15.2 kDa
Purity≥ 95% as determined by SDS-PAGE≥ 97% as determined by SDS-PAGE and RP-HPLC
Activity

≥ 0.9 x 106 IU/mg, calibrated against NIBSC #91/510
Measured in a cell proliferation assay using an IL-3-dependent cell line, TF-1

0.9 – 3.6 x 106 IU/mg, calibrated against NIBSC #91/510
Measured in a cell proliferation assay using an IL-3-dependent cell line, TF-1

Batch specific activity on CoA

Endotoxin level< 1000 EU/mg≤ 50 EU/mg
Intended useIntended for preclinical ex vivo use. Not intended for therapeutic use.Intended for clinical ex vivo use. Not intended for human in vivo application.

Handling Instructions

Reconstitution

Recommended in sterile water to a final concentration of 250 µg/ml.

Shipment

Ambient temperature. Please refer to our Technote Shipment of CellGenix® Preclinical and GMP Cytokines at Ambient Temperatures.

Expiry

≥ 6 months from date of shipping. Please refer to Certificate of Analysis for the exact expiry date.

Storage & Stability

Store lyophilized cytokine at -20°C to -80°C.

Store a 100 µg/ml reconstituted cytokine solution:

• 4 weeks at 2°C to 8°C under sterile conditions after reconstitution. Store in the original container. 

• 4 months at -20°C to -80°C under sterile conditions after reconstitution. Store in 60 µl aliquots in polypropylene cryogenic vials.

Avoid repeated freeze/thaw cycles.