The enclosed ELISA assay kit for anti-PD-1 h-mAb in human serum is based on an enzyme immunoassay (ELISA) between recombinant PD-1 protein (ECD) and a biotinylated anti-PD-1 monoclonal antibody. The method employs the principle of competitive ELISA, allowing quantification of monoclonal anti-PD-1 antibodies in human serum.The assay only involves the following steps:
1. Coat the plate with human PD-1;
2. Add the mixture of your sample and biotinylated anti-PD-1 antibody;
3. Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate;
4. Record the OD readings and analyze the serum concentration.
