Designed to Accelerate Single-Cell Cloning Workflows

Seeding for high clonal efficiency

For 96 and 384 well plates and quality single-project use consumables, we employ the simplest and safest method of single-cell dispensing, with no complex fluidics or mixed samples. VIPS uses low pressure to seed cells in nanoliter droplets into empty wells.

Always in focus

Intelligent Image Analysis

Using advanced image analysis technology, z-stack images are processed to confirm the presence of a single cell.

In-line media dispensing

QC verification process

To assist in the verification of your clonality workflow, VIPS offers a verification methodology option based on rare-event fluorescence imaging.

In-well z-stack imaging

Understanding The ‘Double Lock’ of Clonal Assurance

#1 Single Cell Seeding with In-Well Verification

Operating at a pressure of less than one psi, VIPS gently dispenses cells in nanoliter droplets into a plate well. Crucially, the cell is imaged in the bottom of the dry well as evidence of seeding.Automated imaging in multiple tight z-stack layers of the whole droplet commences with intelligence-based image analysis confirming the existence of a single cell. The well is then filled automatically with media.

#2 Outgrowth and Clonal Characterization

During the clonal growth phase, VIPS performs daily high-clarity whole-well imaging, recording a timeline of well images and performing analysis to measure confluency..Successful clonal outgrowth can be traced back to a single cell to confirm monoclonality.