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홈 > 제품정보 > 시약 > 해외 공식 대리점 > Cusabio > [CSB-E12654r] Rat luteinizing hormone (LH) Elisa kit
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[CSB-E12654r] Rat luteinizing hormone (LH) Elisa kit

제품코드 : CSB-E12654r

제품정보 : Rat luteinizing hormone (LH)

  • Sample Types: serum, plasma
  • Detect Range: 0.3 mlU/ml-60 mlU/ml
  • Sensitivity: 0.15 mlU/ml

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  • 전화번호
    2292-8870

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Product Details

Description

This Rat luteinizing hormone (LH) ELISA Kit was designed for the quantitative measurement of Rat luteinizing hormone (LH) protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 0.3 mLU/mL-60 mLU/mL and the sensitivity is 0.15 mLU/mL.

Alternative NamesLhbLutropin subunit beta ELISA Kit; Lutropin beta chain ELISA Kit; Luteinizing hormone subunit beta ELISA Kit; LH-B ELISA Kit; LSH-B ELISA Kit; LSH-beta ELISA Kit
AbbreviationLH
Uniprot No.P01230
SpeciesRattus norvegicus (Rat)
Sample Typesserum, plasma
Detection Range0.3 mlU/ml-60 mlU/ml
Sensitivity0.15 mlU/ml
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaSignal Transduction
Assay Principlequantitative
MeasurementCompetitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%   
Three samples of known concentration were tested twenty times on one plate to assess. 
Inter-assay Precision (Precision between assays): CV%<15%   
Three samples of known concentration were tested in twenty assays to assess.  
       
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4) 
1:1Average %93 
Range %88-100 
1:2Average %95 
Range %91-101 
1:4Average %94 
Range %85-105 
1:8Average %96 
Range %90-102 
Recovery
The recovery of rat LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange 
Serum (n=5)9893-107 
EDTA plasma (n=4)9691-103 
       
       
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/mlOD1OD2Average  
600.1500.1530.152  
150.3520.3380.345  
4.80.5130.5490.531  
1.20.9851.0020.994  
0.31.4761.4411.459  
02.6792.6542.667  
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs		ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days

Citations

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